Part:BBa_K1951000
Contents
DesA, lysine decarboxylase coding sequence
General information
Lysine descarboxylase (Streptomyces coelicolor) is an enzyme from the lyase family that converts lysine to cadaverine.
The enzyme realizes the carbonyl group of the lysin amino acid.
Cadaverine is a foul-smelling diamine compound produced by the putrefaction of animal tissue. Cadaverine is a toxic[1] diamine with the formula NH2(CH2)5NH2, which is similar to putrescine. Cadaverine is also known by the names 1,5-pentanediamine and pentamethylenediamine.
This enzyme is the first one involved in the production of desferrioxame B which is a well documented siderophore.
Detection method
In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.
Be aware
Cadaverine is toxic in large doses. In rats it has a low acute oral toxicity of 2000 mg/kg body weight, with no-observed-adverse-effect level of 2000 ppm (180 mg/kg body weight/day).
It is a foul-smelling diamine compound.
Design summary
All forbidden restriction sites have been removed.
Codons have been optimized for E.coli to allow a maximal transcription level.
Experiment summary
The following biobrick functionality has been tested by our team in two different composite parts we designed :
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